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Effect of Arbuscular mycorrhizae on Jatropha curcas further its effect on tissue culture and leaf extract for antifungal property

Shankar Ilango*, David Paul Raj

Abstract


Jatropha curcas is significant for its biodiesel and medicinal properties. This study is to compare growth of inoculated Arbuscular Mycorrhizae Fungi (AMF) and control effects on callus development, suspension culture and antifungal activity. Two hundred J. curcas cuttings were grouped into four equal sets and inoculated with AMF of 5g, 10g and15g set with one set kept as control. Micropropogation conducted with MS medium supplemented supplemented sucrose in combination with IBA (Indole 3 butryic acid) and BAB (benzyl adenine). After 6 months sun dried leaves were powdered and treated with 1:1 chloroform methanol solvent. This was column chromotographed with hexane and ethyl acetate (80:20) that separated into four column bands, later analyzed for antifungal activity. In three months root length, total colonization levels and spore number was found to increase according to the amount of AMF applied. The callus growth between the control and treated did not show a significant difference. But in general hypocotyl increased in dry cell weight compared to leaves. The suspension culture growth rate of fresh weight increased till 2 weeks then there was a rapid growth decline. The 6 months AMF treated and control column extracts failed to show any antifungal properties.

Keywords


Arbuscular Mycorrhizae fungi; Jatropha curcas; inoculation; callus; antifungal activity and suspension culture

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References


Ames RN, Reid CPP, Porter LK, Cambaedella C, Hyphal uptake and transport of nitrogen from two 15N-labelled sources by Glomus mosseae, a vesicular-arbuscular mycorrhizal fungus. New Phytol, 1983, 95, 381-396.

Brandon NJ, Shelton HM, Role of vesicular-arbuscular mycorrhizae in Leucaena establishment. Proceedings of the XVII International Grassland congress, 1993, 2064-2065.

Datta, SK, Pandey. RK, Jatopha curcaas: A Promising Crop for New Source of Fuel, Appl Bot Abstr, 1993, 1(2), 108-118.

Escalona M, Lorenzo JC, Gonzaler B, Daquinta M, Gonzaler JL, Desjarodin Y, Borrota CG, Pineapple, (Anunus Comsus L. Merr.) Micropropagation in temporary immersion Systems, Plant Cell Rep, 1999, 18, 743-748.

Fang Yu, Dongyan Zhang B, Fengwu Ai, Lijia An, The accumulation of Isocamptothecin A and B in suspension cell cultures of Camptotheca acuminate, Plant Cell Tiss Org, 2005, 81, 159-163.

Hippolyte I, Marin B, Baccou JC, Jonard R, Growth and rosmarinic acid Production in cell suspension cultures of Salvia officinalis L., Plant Cell Rep, 1992, 11, 109-112.

Igbinosa OO, Igbinosa EO, Aiyegoro OA, Antimicrobial activity and phytochemical screening of stem bark extracts from Jatropha curcas (Linn)., Afric J Pharm Pharmacol, 2009, 3(2), 58–62.

Ilan A, Zliv M, Halevy AH, Propagation and corn development of Brodiaca in liquid culture, Sei Hort, 1995, 63, 101-112.

Irobi ON, Moo-Young M, Anderson WA, Daramola SO, Antimicrobial activity of the bark of Bridelia ferruginea (Euphorbiaceae), Int J Pharmacol, 1994, 34, 87-90.

Kim EK, Hahn EJ, Murthy HN, Paek KKY, High frequency of short multiplication and bulblet formation of garlic in liquid culture, Plant Cell Tiss Org Culture, 2003, 73, 231-236.

Kim SW, In DS, Tae KH, Liu JR, Somatic embryogenesis and plant regeneration in leaf and petiole explant cultures and cell suspension cultures of Pinellia tripartite, Plant Cell Tiss Org Culture, 2005a, 80, 267-270.

Kim SW, In DS, Choi PS, Liu JR, Plant regeneration from immature zygotic embryo-derived embryogenic calluses and cell suspension cultures of Catharanthus roseus, Plant Cell Tiss Org, 2005, 76, 131-135.

Kobayashin AK, Vieira LGE, Establishment of an in vitro system for studies on the induced resistance of cotton to Xanthomonas Campestris Pv. Malvacearum, Pesq Agropec bras Brasilia, 2000, 35(4), 719-725.

Monacelli B, Pasqua G, Cuteri A, Varusio A, Botta B, Monache GD, Histological study of callus formation and optimization of cell growth in Taxus Baccata, Cytobios, 1995, 81, 159-170.

Mudalige RG, Longstreth D, Effects of Salinity on photosynthetic characteristics in Photomixotrophic, cell suspension cultures from Alternanthera, philoxeroides, Plant Cell Tiss Org, 2006, 84, 301-308.

Munch E, Kielfer J, Purging nut (Jatropha curcas L.); multi-use plant as a source of fuel in the future. Schriftenreihe der. Schriftenreiheder GTZ. 1989, No. 209, pp. 1-32

Nakamura T, Islinkawa M, Transformation of suspension cultures of bromegrass (Bromus inermis) by Agrobacteruim tumefactions. Plant Cell, Tiss Org, 2006, 84, 293-299.

Pathirana R, Eason JR, Establishment and characterization of a rapidly dividing diploid cell suspension culture of Arabidopsis thaliana suitable for cell cycle synchronization, Plant Cell Tiss Org, 2006, 85, 125-136

Rao AQ, Sarfraz HS, Sadqib SM, Bokhari S, Tayyab YAH, Rizuddin S, Somatic embrogenesis in wild relatives of cotton (Gossipium spp.). J Zhejiang Univ Sci, 2006, B 291-298.

Risika G, Mudalige, Logstregth DJ, Effect of salinity on photosynthetic characteristics in photomixtrophic cell-suspension cultures from Alternanthera philoxeroides, Plant cell Tiss Org, 2006, 84, 301-308.

Sanders FE, Sheikh NA, The development of vesicular-arbuscular mycorrhizal infection in plant root systems, Plant and Soil, 1983, 71, 223-246.

Srivastava NK, Basu M, Occurrence of vesicular arbuscu lar mycorrhizal fungi in some medicinal plants. In: Adholeya, A., Singh, S. (ed.), Mycorrhizae: Biofertilizers for the Future. Third National Conference on Mycorrhiza, TERI, Delhi, India, 1995, 58–61.

Sujatha M, Mukta, Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas, Plant cell Tiss Org, 1996, 44, 135-141.

Tewari DN, Jatropha and biodiesel. Ocean Books, Ltd. New Delhi, 2007.

Zhong JJ, Konstantinov KB, Toshida T, Computeraided on line mnitoring of physiological variables in suspended cell cultures of a Perillafrutescens in a bioreactor, J Ferment Bioeng, 1994, 77: 445-447.


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