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Optimization of conditions for vegetative propagation of Taxus baccata by shoot cuttings: Effects of IBA, growth stage and seasonal differences

Saumitro Das, Jha L.K.


Taxus baccata is a highly valued medicinal tree, but overexploitation and inadequate natural regeneration, in addition to habitat destruction, has increased the threat of extinction. Various researchers have proposed shoot cuttings as cost effective method for propogation of this noble tree species because its regeneration through seed is very poor. The present study was conducted to investigate the effect of growth stage of shoots and IBA concentrations on rooting during four different seasons. Regardless of season, rooting was influenced by growth stage, IBA treatment and their interaction. Best rooting performance was exhibited by cuttings planted during rainy season. Cuttings of second year shoot scored the highest rooting percentage in all seasons (47.3% in rainy > 26.9% in autumn >10.2% in winter) except in spring where third year shoot’s cutting (22.8%) had the highest value. Rooting of cutting was enhanced by IBA treatment. Highest rooting percentage during spring (22.9%) and rainy season (44.0%) was achieved with low concentration of IBA (1000 ppm) whereas during autumn (25.9%) and winter season (28.4%) rooting was induced by high concentration of IBA (4000-5000 ppm). The interaction between growth stage and IBA treatment was significant for all season except winter.


IBA, growth stage, rooting percentage, seasonal difference, shoot cuttings, Taxus baccata

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