Study of in vitro antibacterial activity of leave extract of Citrus maxima

Amiya Kumar Prusty*, Saroja Kumar Patro


The objective of the study was to evaluate the antibacterial activity of the ethanolic extracts of leave of Citrus maxima (Burm.) Merr. on Staphylococus aureus, Bacillus subtilis, Escherichia hermanii and Pseudomonas aeruginoss. The ethanolic leave extract of Citrus maxima (Burm) Merr. was prepared by percolation method followed by  antimicrobial susceptibility test using disc diffusion method. The whatman-1 filter paper discs of 6mm sizes impregnated with the extract were placed on agar plates seeded with bacterial cultures of 0.5 McFarland standards. The antibacterial activities were assessed by the presence or absence of inhibition zones after incubating the plates at 370C for 24 hours. Minimum inhibitory concentration (MIC) of ethanolic extract for the selected pathogens were determined by broth macro dilution method. Maximum zone of inhibition in antibacterial susceptibility test was shown by Staphylococus aureus. MIC value of the extract for Staphylococus aureus and Bacillus subtilis (20µg/ml) was found to be lower than Escherichia hermanii (30µg/ml) and Pseudomonas aeruginoss (40µg/ml). The Leave extract of Citrus maxima showed significant antibacterial activity against Escherichia hermanii, staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginoss.


Citrus Maxima; Ethanol extract; Antimicrobial activity; MIC.

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