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Impact of culture media and growth hormones on callus induction and root organogenesis in cotyledon segment of Anacardium occidentale L.

Sija S. L.*, Potty V. P., Santhoshla P. S.

Abstract


The present study aimed to determine the optimal cultural conditions for the initiation of callus and root organogenesis in cotyledon segment of cashew (Anacardium occidentale L). One of the major problems in in vitro propagation of cashew is the blackening/browning of tissue, which is mainly due to the exudation of phenolic compound from the cut end of explants. In the current study, inhibitory effects of different treatments such as adding activated charcoal, polyvinylpyrrolidone (PVP), ascorbic acid to the medium, continuous subculture passages or dark incubation were investigated against browning problem of A.occidentale. Adding a combination of 3g/L activated charcoal, 75 mg/L ascorbic acid and 2.5g/L polyvinylpyrrolidone (PVP) to the Murashige and Skoog (MS) basal medium and Woody Plant (WP) medium was found as the most effective treatment. The cotyledonary explants, excised from mature seeds were screened at various concentration and combinations of BAP and 2,4-D in the range 0-75mg/L for callus production. The developed callus in this medium was pale yellow, light to dark brown, semi friable, compact and slow growing. The result also revealed that WP medium with high concentration of 2,4-D (50 mg/L) and BAP (50 mg/L) exhibited profuse root organogenesis than MS medium.

Keywords


Anacardium occidentale; activated charcoal (AC); polyvinylpyrrolidone (PVP); growth regulators; callus induction; root organogenesis

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References


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