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Effect of Arbuscular mycorrhizae on Jatropha curcas further its effect on tissue culture and leaf extract for antifungal property

Shankar Ilango*, David Paul Raj

Abstract


Jatropha curcas is significant for its biodiesel and medicinal properties. This study is to compare growth of inoculated Arbuscular Mycorrhizae Fungi (AMF) and control effects on callus development, suspension culture and antifungal activity. Two hundred J. curcas cuttings were grouped into four equal sets and inoculated with AMF of 5g, 10g and15g set with one set kept as control. Micropropogation conducted with MS medium supplemented supplemented sucrose in combination with IBA (Indole 3 butryic acid) and BAB (benzyl adenine). After 6 months sun dried leaves were powdered and treated with 1:1 chloroform methanol solvent. This was column chromotographed with hexane and ethyl acetate (80:20) that separated into four column bands, later analyzed for antifungal activity. In three months root length, total colonization levels and spore number was found to increase according to the amount of AMF applied. The callus growth between the control and treated did not show a significant difference. But in general hypocotyl increased in dry cell weight compared to leaves. The suspension culture growth rate of fresh weight increased till 2 weeks then there was a rapid growth decline. The 6 months AMF treated and control column extracts failed to show any antifungal properties.

Keywords


Arbuscular Mycorrhizae fungi; Jatropha curcas; inoculation; callus; antifungal activity and suspension culture

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References


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