Cr+6- induced growth, biochemical alterations and Chromium bioaccumulation in Cassia tora (L.) Roxb.

Priyanka Jena, Chinmay Pradhan, Hemanta Kumar Patra*

Abstract


The present in vivo pot culture experiments showed hexavalent Chromium (Cr+6) induced growth, physiological and biochemical alterations and Chromium bioaccumulation in 60 days Cassia tora (L.) Roxb. (Coffe Pod) plants. Root and shoot length, fresh and dry matters considerably decreased with increasing concentration of Cr+6. However, in 10 ppm shoot dry weight increased. High dose Cr+6 application showed decreased chlorophyll, protein and catalase activity. The activity of Catalase increased with 10 ppm of Cr+6 supply to the soilwhich declined with 50 ppm of Chromium in leaves. Total sugar, reducing sugar, starch and total carbohydrate contents also decreased with increase Cr+6 concentration. Proline and peroxidase activities increased with increasing concentration of Cr+6. Chromium bioaccumulation was more in roots than leaves and stems. Bio-Concentration Factor (BCF), Total Accumulation Rate (TAR) indicated highest values i.e. 0.051 and 0.070 respectively in 50 ppm whereas Transportation Index (TI) was highest (0.814) in 10 ppm of Cr+6. The results of the present study revealed that the phytoremediation ability of Coffe pod plants growing under wild conditions can be used to combat Cr +6 stress in chromium contaminated areas.

Keywords


Biochemical alterations; Cassia tora; Cr+6application; Cr-bioaccumulation plant growth

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References


Arnon D.I, “Copper enzymes in chloroplast polyphenol oxidase in Beta vulgaris” Plant Physiol, 24 (1949): 1-15.

Bates L.S, R.P Waldren, I.D Teare, “Rapid determination of free proline for water stress studies”, Plant and Soil, 39(1973): 205-207.

Bonet A., C.H Poschenrieder, J. Barcelo, “Chromium III-iron interaction in Fe-deficient and Fe-sufficient bean plants. I. Growth and nutrient content”, J. Plant Nutr, 14(1991):403-41.

Bonnet M., O. Camares, P. Veisseire, “Effect of zinc and influence of Acremoniumlolii on growth parameters, chlorophyll a fluorescence and antioxidant enzyme activities of ryegrass (Lolium perenne L. cv apollo)”, J. Exp. Bo, 51 (2000:) 945–953.

Chance B, A. C Maehly, “Assay of catalase and peroxidase", Methods Enzymol, 2(1995): 764-775.

Chaturvedi N, N.K Dhal, H.K Patra, “EDTA and citric acid-mediated phytoextraction of heavy metals from iron ore tailings using Andrographis paniculata: a comparative study”, Int. J. Min. Reclam. Env, 29(1) (2015): 33–46.

Datta J.K, A. Bandhyopadhyay, A. Banerjee, N.K Mondal “Phytotoxic effect of chromium on the germination, seedling growth of some wheat (Triticum aestivum L.) cultivars under laboratory condition”, J Agr. Tech, 7(2) (2011): 395- 402.

Erenoglu B.E, H.K Patra, H. Khodr, V. Römheld, N.V Wirén, “Uptake and apoplasmic retention of EDTA and phytosiderophore-chelated chromium (III) in maize” J Plant Nutr and Soil Sci, 170(6) (2007): 788–795.

Ghosh M., S.P Singh, “A review on phytoremediation of heavy metals and utilization of its by products”, Appl. Ecol. Env Res, 3(1) 2005a: 1–18.

Ghosh M., S.P Singh, “Comparative uptake and phytoextraction study of soil induced chromium by accumulator and high biomass weed species”, Appl. Ecol. Env Res, 3(2) 2005b, 67–79.

Iqbal M.Z, K. Rahmati, “Tolerance of Albizia lebbeck to Cu and Fe application, Ekologia, 111992,427-430.

Labra M., E. Gianazza, R. Waitt, I. Eberini, A. Sozzi, “Zea mays L. protein changes in response to potassium dichromate treatments”, Chemosphere, 62 (2006):, 1234-1244.

Mohanty M.., C. Pradhan, H.K Patra, “Chromium translocation, bioconcentration and its phytotoxic impacts in in vivo grown seedlings of Sesbania sesban L. Seedlings”, Acta Biol Hung, 66(1) 2015, 80-92.

Mohanty M., H. K. Patra, “Attenuation of Chromium Toxicity by Bioremediation Technology”, Rev.Env Cont Toxicol, 210 (2011) 1-34.

Mohanty M., H.K Patra, “Phytoremediation Potential of Paragrass – An in situ Approach for Chromium Contaminated Soil”, Int J Phytoremed, 14(8) (2012), 796-805.

Nelson N., “Photometric adaptation of Somogyi method for the determination of glucose”, J. Biol. Chem, 153 (1944): 275-380.

Nagoor S., “Physiological and biochemical responses of Cereal seedling to graded levels of heavy metals. II. Effect on protein metabolism in maize seedlings”, Adva. Plant Sci. 12 (1999):425-433.

PandaS. K, H. K Patra, “Physiology of Chromium Toxicity in Plants- A Review”. Plant Physiology & Biochemistr, 4 (1997)10-17.

Patra H.K, D. Mishra, “Phytophosphatase, peroxidase and Polyphenol oxidase activities during leaf development and senescence”, Plant Physiol, 63(1979): 318-323.

Patra H. K, M. Kar, D. Mishra, “Catalase activity in leaves and cotyledons during plant development and senescence”, Biochem Physiol Pflanzen, 172 (1978): 385-390.

Paiva H. N, J. G Carvalho, J. O Siqueira, “Efeito de Cd, Ni, Pb e Zn sobre mudasdecedro (Cedrela fissilis Vell.) e de iproxo (Tabebuia impetiginosa (Mart.Standley)emsolu¸c˜aonutritiva”, Revista Árvore, 24 (2000): 369–378.

Porra R.J “The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b”, Photosynth Res, 73(1-3) (2002): 149 -156

Prassad T. K “Mechanisms of chilling-induced oxidative stress injury and tolerance in developing maize seedlings: changes in antioxidant system, oxidation of proteins and lipids, and protease activities”, Plant J. 10 (1996): 1017–1026.

Romero-Puertas M. C., J. M. Palma, A. Del Rio, L.M. Sandalio, “Cadmium causes the oxidative modification of proteins in pea plants”, Plant Cell Environ, 25 (2002): 677-686.

Salt, D. E, R. D. Smith, I. Raskin, “Phytoremediation”, Annu. Rev. Plant Physiology, 49(1998): 643-68.

Siringoringa H. H, “The role of some urban forest plants in adsorbing lead particulates”, Bull Penelitian Hutan, 622 (2000):1-16.

Shirbhate N., S.N. Malode, “Phytoremediation potential of Cassia tora (L.) Roxb. To remove heavy metals from waste soil, collect from Sukali compost and Landfil Depot, Amaravati (M.S.)”, Gobal Jour. Bio.Sc. and Biotech, 1(2012): 104-109.

Yoshida S., D. A. Forno, J. H. Cock, K.V. Gome, “In: Laboratory manual for physiological studies of rice, the International Rice Research Institute, Lose Banos, Philippines”, 81(1972).

Wong, M. H, A. D. Bradshaw, “A comparison of the toxicity of heavy metals, using root elongation of rye grass, Lolium perenne”, New Phytologist, 91(1982):255-261.

Zayed A. M, N. Terry, “Chromium in the Environment: Factor affecting biological remediation”, Plant and Soil, 249 (2003): 139–156.

Zeid I. M., “Response of Phaseolus Vulgaris to chromium and cobalt treatments”, Biol.Plant, 44(2001): 111-115.

Zurayk R., B. Sukkariyah, R. Baalbaki, D.A. Ghanem, Water Air and Soil Pollut, 139 (1-4) (2002): 355– 364




DOI: https://doi.org/10.21746/aps.2016.07.001

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