Allelopathic effect of Pinus roxburghii on an understorey plant, Bidens pilosa

Neel Kant Sharma*, Daizy R. Batish, Harminder Pal Singh, R. K. Kohli


Allelopathic effect of Pinus roxburghii was studied on Bidens pilosa through laboratory and greenhouse experiments. The aqueous extracts of green needles, needle litter and bark were found to inhibit germination and initial growth of B. pilosa in laboratory bioassays. The inhibitory effect on germination root length, shoot length and biomass was also observed on amendment of powdered needle litter in soil in greenhouse pots. The inhibitory effect increased with increase in conc. of extracts or amount of litter amended. The amended soil was found to be rich in phenolics, the known potent allelochemics. The study indicates that such interactions may also be operational in forests of P. roxburghii.


Allelopathy; Aqueous extracts; Needles; Phenolics; Pinus roxburghii

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Blum, U., S.R. Shafer and M.E. Lehman. “Evidence for inhibitory allelopathic interactions involving phenolic acids in field soils: concepts vs. an experimental model.” Critical Reviews in Plant Sciences 18 (1999): 673–693.Print.

Cheng, H.H. “A conceptual framework for assessing allelochemicals in the soil environment.” In: S.J.H. Rizvi and V. Rizvi (ed.) Allelopathy: Basic and Applied Aspects. Chapman and Hall Publisher, UK (1992): 21-29. Print.

Gallet, C. “Allelopathic potential in bilberry-spruce forests, influence of phenolic compounds on spruce seedlings.” Journal of Chemical Ecology 20 (1994): 1009-1024.Print.

Jobidon, R. “Allelopathic potential of coniferous species to old- field weeds in Eastern Quebec.” Forest science 32 (1986): 112-118.Print.

Kato-Noguchi, H., Y. Fushimi and H. Shigemori. “An allelopathic substance in red pine needles (Pinus densiflora).” Journal of Plant Physiology 166 (2009): 442-446.Print.

Kil, B. S. “Effect of pine allelochemicals on selected species in Korea.” In: S.J.H. Rizvi and V. Rizvi (ed.) Allelopathy: Basic and Applied Aspects. Chapman and Hall Publisher, UK (1992): 205-241.Print.

Kil, B.S., and Y.J. Yim. “Allelopathic effects of Pinus densiflora on undergrowth of Red Pine Forest.” Journal of Chemical Ecology 9 (1983): 1135-1151.Print.

Kil, B.S., D. Y. Kim, Y.S. Kim and S.Y. Lee. “Phytotoxic effects of naturally occurring chemicals from Pinus koraiensis on experimental species.” Korean Journal of Ecology 14 (1991):149-157.Print.

Kim, Y.O., H.J. Lee and N. K. Chang. “Effects of Pinus rigida allelochemicals on isozyme activities during seed germination of Cassia mimosoides var. nomame.” Korean Journal of Ecology 20 (1997):103-109.Print.

Lodhi, M.A.K., and K.T. Killingbeck. “Effect of pine produced chemicals on selected understory species in a Pinus ponderosa community.” Journal of Chemical Ecology 8 (1982): 275-283.Print.

Melkania, N.P., J.S. Singh and K.K.S. Bisht. “Allelopathic potential of Artemisia vulgaris L. and Pinus roxburghii Sargent: A bioassay study.” Proceedings of Indian National Science Academy Part B 48 (1982): 685—688.Print.

Muller, C.H. “Allelopathy as a factor in ecological process." Vegetation 18 (1969):348-357.Print.

Pellissier, F., and X.C. Souto. “Allelopathy in northern temperate and boreal semi-natural woodland.” Critical Reviews in Plant Sciences 18.5 (1999): 637–652.Print.

Pérez-Corona, M.E., P. De lash eras and B. R. Vazquez de Aldana. “Allelopathic potential of invasive Ulmus pumila on understory plant species.” Allelopathy Journal 32 (2013): 101-112.Print.

Refifa, T., H. Chahdoura, G. Flamini, K. Adouni, L. Achour and A. Helal. “Allelopathic potential of Pinus halepensis needles.” Allelopathy Journal 38.1 (2016): 103-124.Print.

Reigosa, M.J., X.C. Souto and L. Gonzalez. “Allelopathy research: Methodological, ecological and ecolutionary aspects.” In: Allelopathy, Field observations and Methodology. Narwal, S.S. and Tauro, P. (Eds.). Scientific Publishers, Jodhpur 1 (1996): 213-231.Print.

Rice, E.L. Allelopathy, 2nd edition, Academic Press, Orlando, Florida, USA (1984). Print.

Singh, H.P., D. R. Batish and R. K. Kohli. “Allelopathic effect of Leucaena leucocephala on Zea mays.” Journal of Tropical Forest Science 11.4 (1999a): 801-808. Print.

Singh, H.P., R. K. Kohli, D. R. Batish and P. S. Kaushal. “Allelopathy of Gymnospermous trees.” Journal of Forest Research 4 (1999b): 245-254.Print.

Swain, T. and W. E. Hillis. “The phenolic constituents of Prunus domestica I.-The quantitative analysis of constituents.” Journal of the Science of Food and Agriculture 10 (1959): 63-68. Print.

Taylor, R.J. and D.C. Shaw. “Allelopathic effects of Engelmann spruce bark stibenes and tannin-stibene combinations on seed germination and seedling growth of selected conifers.” Canadian Journal of Botany 61.1 (2011): 279-289.Print.

Valera-Burgos, J., M. C. Diaz-Barradas and M. Zunzunegui. “Effects of Pinus pinea litter on seed germination and seedling performance of three Mediterranean shrub species.” Plant Growth Regulation 66 (2012): 285–292.Print.

Whittaker, R.H. “The biochemical ecology of higher plants.” In: E. Sondheimer and J.B. Simeone (Eds.) Chemical Ecology, Academic Press, N.Y. (1970): 43-70. Print.

Williams, R. D. and R. E. Hoagland. “The effects of naturally occurring phenolic compounds on seed germination.” Weed Science 30 (1982): 206-212.Print.

Wu, H.W., J.B. Zhang, R. Stanton, M. An, D.L. Liu and D. Lemerle. “Allelopathic effects of Eucalyptus dundasii on germination and growth of ryegrass and barley grass.” Allelopathy Journal 28 (2011): 87–94. Print.

Zen, R.S. “Allelopathy: the solution is indirect.” Journal of Chemical Ecology 40 (2014): 515- 516.Print.


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