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Hepatoprotective efficacy of ethanolic extracts of rhizome Curcuma amada Roxb. In experimental rats

Ramnath V., Maria Caroline Rebellow M., Seethalakshmi S.


The scope of this study is to evaluate the hepatoprotective efficacy of rhizome Curcuma amada Roxb. (CAR) in CCl4 induced hepatotoxicity in rats. Male Albino Wister rats were divided into six groups (n=6). Group I served as the normal control group and received olive oil (i.p. 0.5 mL/kg b.w.) as a vehicle. Group II served as high dose group and received 400mg/kg b.w CAR. Group III served as the carbon tetrachloride (CCl4) group and received CCl4 (i.p., 0.1 mL/kg b.w., 50% CCl4 in olive oil). Groups IV–VI served as the treatment groups, and they received CARdissolved in distilled water orally at dose levels of 100, 200, and 400 mg/kg b.w., respectively, with CCl4 (i.p., 0.1 mL/kg b.w., 50% CCl4 in olive oil). All the groups were given the respective dosages twice a week for 28 days. The result of the marker enzymes AST, ALT, ALP and TBARS in the serum sample revealed an appreciable increase in groups IV, V and VI with respect to CCl4 treated group. This confirmed the hepatoprotective nature of CAR there by deactivating the phase II detoxifying enzymes, preventing the formation of free radical and protecting the cell membrane from degeneration. The nonenzymatic antioxidants pattern of GSH, GPX and GST showed decreased levels with respect to group III. This confirmed that CAR has induced the GSH antioxidant system by increasing cellular defense against reactive free radicals and other oxidative species. The histological architecture of liver sections in Group-IV–VI showed more or less normal lobular pattern with mild degrees of fatty change, necrosis and lymphocyte infiltration almost comparable to those of control group. These results act as a supporting evidence to exhibit the hepatoprotective nature of CAR.


Curcuma amada Roxb., Hepatoprotective, CCl4.

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X Shen, Tang Y, Yang R, Yu L, Fang T, and Duan JA. “The protective effect of Zizyphus jujube fruit on carbon tetrachloride induced hepatic injury in mice by anti-oxidative activities”. J Ethnopharmacol 122.3 (2009): 555-560.

JA Patel, and Shah US. “Hepatoprotective activity of Piper longum traditional milk extract on carbon tetrachloride induced liver toxicity in Wistar rats”. Bol Latinoam Caribe Plant Med Aromat 8.2 (2009): 121-129.

B Halliwell, and Gutteridge JMC.”Free radicals in biology and medicine”. Nueva York: Oxford University Press (2007).

A Amin, Mahmoud-Ghoneim D, and Zizyphusspina-christi. “Protects against carbon tetrachloride induced liver fibrosis in rats”. Food Chem Toxicol 47.8 (2009): 2111-2119.

J Ananthi, Prakasam A, and Pugalendi KV. “Antihyperglycemic activity of Eclipta alba leaf on alloxan induced diabetic rats”. Yale J Biol Med 76.3 (2003): 97-102.

F Stickel, and Schuppan D. “Herbal medicine in the treatment of liver diseases”. Dig Liver Dis 39 (2007): 293-304.

KR Kirtikar, and Basu, BD.”Indian medicinal plants”. Dehradun: Bishen Singh & Mahendra Paul Singh (2005): 2078-2079.

A Chatterjee.” The treatise of indian medicinal plants”. New Delhi, India: CSIR Publication (1997): 69-70.

M Bathori, Toth I, Szendrei K, and Reisch J. “Ecdysteroids in Spinacea oleracea and Chenopodium bonus-Henericus”. Phytochemistry 21 (1982): 236-238.

KH Janbaz, and Gilani AH. “An assessment of the potential of protopine to inhibit microsomal drug metabolizing enzymes and prevent chemical induced hepatotoxicity in rodents”. Pharmacological Research 38 (1998): 215-218.

CD Klaassen. “The pharmacological basis of therapeutics”. 7th Edition, New York, Toronto, London, Macmillan (1985):1635-1636. Klaui. “Industrial and commericial uses of carotenoids”. Carotenoid Chemistry and Biochemistry Briton G and Godwin TW (1982): 309-317.

Y Oshima, Kawakami Y, Kiso Y, Hikino H, Yang L, and Yen KY. “Antiihepatotoxic principles of Aeginatia indica herbs”. Syhoyakugaku Zasshi 30 (1984): 198 – 200.

SC Lin, YH Chen, CY Hsu, and SH.” The Hepatoprotective and therapeutic effect of propolis ethanol extract on chronic alcoholinduced liver damage”. Am. J. Chin. Med 25 (1997): 325 – 332.


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