Comparitive Phytochemical, antimicrobial and antioxidant studies on two Lamiaceae Ocimum species of O. tenuiflorum L. and O. tenuifolium L. from Visakhapatnam, Andhra Pradesh, India.

Madhuri Tulugu, Seetha Lakshmi B., Sujatha B.

Abstract


Ocimum tenuiflorum and Ocimum tenuifolium are the most popular traditional plants in the history of India. Present investigation is to compare the phytochemical, antimicrobial and antioxidant parameters of organic solvent extracts of hexane, chloroform and methanol. Qualitative phytochemical tests were used to detect the presence of terpenoids, flavonoids, tannins, saponins, glycosides and phenols, while two quantitative methods; Ferric Reducing Antioxidant Power (FRAP) and diphenyl -1, 2-picryl hydrazyl (DPPH) were used to determine the antioxidant potential. The antimicrobial activity was determined by Agar well diffusion method and was screened against seven micro organisms. The highest antimicrobial, antioxidant activities were observed in the methanol extracts of O. tenuiflorum when compared with O. tenuifolium. Plant species. While the hexane extract showed the least activity irrespective of the method used. The presence of aromatic active phytochemical substances with antioxidant activities may provide substantial basis for the use of these plants in ethnomedicine.


Keywords


Phytochemical analysis; Anti-microbial activity; Ocimum tenuiflorum; Ocimum tenuifolium

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References


Atal, C.K. and B.M. Kapoor, “Cultivation and Utilization of Medicinal Plants.” (1989): United Printing Press, New Delhi, India.

Bhargava, K.P. & N. Singh, “Antistress activity of Ocimum sanctumLinn.”. Indian Journal of Medical Research 73. (1981): 443-451. PMid:7275241

Carovic K., Liber Z., Javornik B., Kolak I. and Z. Satovic “Genetic relationships within basil (Ocimum) as revealed by RADP and ALP markers”, Acta Hort (ISHS), 760. (2006):171–178.

Charles D.J. and J.E. Simon “Comparison of extraction methods for the rapid determination of essential oil content and composition of basil (Ocimum spp.)” J Am Soc Hort Sci, 115. (1990):458–462.

Chang J.D., Mantri N., Sun B. Jiang L. Chen P. and B. Jiang et al., “Effects of elevated CO2 and temperature on Gynostemma pentaphyllum physiology and bioactive compounds.” J. Plant Physiol. 19. (2016): 41–52. doi: 10.1016/j.jplph.2016.02.020.

Cutler H.G. and S.J. Cutler “Biologically Active Natural Products” Agrochemicals (1999): Boca Raton, FL: CRC Press.

Devi, P.U. “Radioprotective, anticarcinogenic and antioxidant properties of the Indian holy basil, Ocimum sanctum (Tulasi).” Indian Journal of Experimental Biology 39. (2001): 185-190.

Godhwani S., Godhwani JL., and D. S. Vyas Ocimum sanctum an experimental study evaluating its anti inflammatory, analgesic and antipyretic activity in animals.” Journal of Ethnopharmacology 21. (1987):153-163. http://dx.doi.org/10.1016/0378-8741(87)90125-5

Gupta MP., Solis PN., Calderon AL., Guionneau-SinclairF., CorseaM. and C.Galdemos “Medical ethnobotany of the tribes of bocas del toro, panama.”J Ethnopharmacol 96. (2005): 389-401, en.

Hawkey P., and D.. Law Medical Bacteriology. 2. (2004): Oxford University Press, New York.

Jiang B., Mantri N., Hu Y., Lu J., Jiang W., and H. Lu “Evaluation of bioactive compounds of black mulberry juice after thermal, microwave, ultrasonic processing and storage at different temperatures.” Food Sci. Technol. Int. 21 (2015): 392–399. doi: 10.1177/1082013214539153

Jiang W., Jiang B., Mantri N., Wu Z., Mao L., Lu H., et al., “Comparative ecophysiological analysis of photosynthesis, biomass allocation, polysaccharide and alkaloid content in three Dendrobium candidum cultivars.” Plant Omics J 7. (2014): 117–122.

Jirovetz L. and G. Buchbauer “Analysis, chemotype and quality control of the essential oil of a new cultivated basil (Ocimum basilicum L.) plant from Bulgaria.” Sci Pharm(2001): 69. 85-89.

Joshi, RK. “Chemical composition, in vitro antimicrobial and antioxidant activities of the essential oils of Ocimum gratissimum, O. Sanctum and their major constituents.” Indian Journal of Pharmaceutical Sciences 75. (2013): 457-462. http://dx.doi.org/10.4103/0250-474X.119834 PMid:24302801 MCid: PMC3831728

Karthikeyan K., Ravichadran P., and S. Govindasamy “Chemopreventive effect of Ocimum sanctum on DMBA-induced hamster buccal pouch carcinogenesis.” Oral Oncology 35. (1999): 112-119. http://dx.doi.org/10.1016/S1368-8375(98)00035-9

Kathiresan K., Guanasekan P., Rammurthy N., and S. Govidswami “Anticancer activity of Ocimum sanctum.” Pharmaceutical Biology 37. (1999): 285-290. http://dx.doi.org/10.1076/phbi.37.4.285.5801

Kunwar, R.M. and N. Adhikari “Ethanomedicine of dolpha district Nepal.” The plants their vernacular names and uses. Lyonia 8. 2005: 43-49.

Mandave P., Pawar P., Ranjekar P. Mantri N. and KuvalekarA. “Comprehensive evaluation of invitro anti oxidantactivity, tota lphenols and chemical profiles of two commercially important strawberry varieties.” Sci. Hortic. 172. (2014). 124–134.doi:10.1016/j.scienta.2014.03.002

Hall, M.G. British Journal of Clinical Pharmacology 52. 2. (2001): 169. doi:10.1046/j.0306-5251.2001.01421.x; PMid:11488774 PMCid:2014534

Mantri N. Olarte A. LiCG. Xue C. and ECK. Pang “Fingerprinting the asterid species using subtracted diversity array reveals novel species-specific sequences.” PLoS ONE (2012): 7. e34873. doi: 10.1371/journal.pone.0034873

Mathela CS. NarwalSS and P. Tauro “Allelochemicals in medicinal and aromatic plants, in Allelopathy in Agriculture and Forestry, eds.” and Jodhper: Scientific Publishers (1991): 213-228.

Manosroia J., Dhumtanoma P., and A. Manosroia “Anti-proliferative activity of essential oil extracted from Thai medicinal plants on KB and P388 cell lines.” Cancer Lett 235. (2006): 114-120.

Mota MLR.Thomas G. Barbosa and JM. Filho “Anti-inflammatory actions of tannins isolated from the bark of Anacardium occidentale (L).”Ethnopharmacol 13. (1985): 289-300 en.

Mondal S. Mahapatra SC. Mirdha BR., and SN. Naik “Antimicrobial activity of essential oils obtained from fresh and dried leaves of Ocimum sanctum L. against enteric bacteria and yeast.” Acta Horticulturae756. (2007): 267-270.

Mukherjee R. Das PK. and Ram GC. “Immunotherapeutic potential of Ocimum sanctum Linn. bovine subclinical mastitis.” Research in Veterinary Science 79. (2005): 37-43. http://dx.doi.org/10.1016/j.rvsc.2004.11.001 PMid:15894022

Niu L, Mantri N.Li CG., Xue C. and ECK. Pang “Array- based techniques for finger printing herbal plants.” BMCChin.Med. 6.18. (2011): doi: 10.1186 / 1749-8546 -6-18

Pandey BP. Economic Botany Ramnagar, New Delhi: Chand and Company Ltd., (1990): 294.

Prashar, R., Kumar A., Banerjee S. and Rao AR. “Chemopreventiveaction by an extract from Ocimum sanctum on mouse skinpapillomagenesis and its enhancement of skin glutathione-S-transferase activity and acid soluble sulfhydryl level.” Anticancer Drugs. 5. (1994): 567-572.

Pattanayak P., Behera P., Das D. and SK. Panda “Ocimum sanctum L. A reservoir plant for therapeutic applications: an overview. Pharmacogn. Rev.” 4. (2010): 95. doi: 10.4103/0973- 7847.65323

Pattanayak P. and Gupta N. “Therapeutic uses of Ocimum sanctum Linn. A reservoir plant for therapeutic applications: An overview. Phoog. Rev.” 4. (2005): 95-105.

Rahman MS. KhanMMH. and Jamal MAHM. “Anti-bacterial evaluation and minimum inhibitory concentration analysis of Oxalis corniculata and Ocimum sanctum against bacterial pathogens.” Biotechnology 9. (2010): 533-536.

Rahman MS. SalehinM.F. and Jamal MAHM. ParvinA. and Alam MK. “Anti-bacterial activity of Argemone Mexicana L. againt water borne microbes.” Res. J. Med. Plant 5. 2011: 621-626.

Ryan KJ. Sherris JC. Ray GC.“Normal Microbial flora.”Medical Microbiology, Fourth. editor(2004). McGraw Hill, USA.

Shimeld LA. “Essential of Diagnostic Microbiology.” International Thomas Publishing Company, USA; (1998).

Siddiqui YH. AraG. BegT. and AfzalM. “Antigenotoxic effect of Ocimum sanctum L. extract against cyproterone acetate induced genotoxic damage in cultured mammalian cells.” Act Biol. Hungerica, 58. (2007): 397-409.

Siddiqui HH. “Safety of herbal drugs- an overview.” Drugs NewsViews, 1. (1993):7-10.

Singh V. Amdekar S. and Verma O. “Ocimum Sanctum (tulsi): Biopharmacological Activities.” Webmed Central Pharmacol. 1. (2010): WMC001046.doi: 10.9754/journal.wmc.2010.001046

Sun X. MantriN.GeJ.DuY.LuJ.JiangW.et.al., “Inhibition of plant pathogens invitro and invivo with essential oil and organic extracts of Torreya grandis ‘Merrilli’aril.” Plant OmicsJ 7. (2014): 337–344.

YangSS. CraggGM. NewmanDJ. and BaderJP.Journal of Natural Products 64. 2. (2001): 265. doi:10.1021/np0003995; PMid:11430019

Seth SD. Johri N. and Sundaram KR. “Antispermatogenic effect of Ocimum sanctum.” Indian Journal of Experimental Biology, 19. (1981): 975-976. PMid:7309144

Singh S. and Majumdar DK. “Evaluation of anti-inflammatory activity of fatty acids of Ocimum sanctum fixed oil.” Indian Journal of Experimental Biology 35. (1997): 380-383. PMid:9315239

Singh S. Malhotra M. and Majumdar DK. “Antibacterial activity of Ocimum sanctum L. fixed oil.” Indian Journal of Experimental Biology, 43. (2005): 835-837.PMid:16187537

Sirkar NN. “Pharmacological Basis of Ayurvedic Therapeutics.” In: C. K. Atal & B. M. Kapoor (Eds.), Cultivation and utilization of medicinal plants. (1989): New Delhi: PID, CSIR.

Victor Njoku O. and Chidi Obi.“Phytochemical constituents of some selected medicinal plants.:African Journal of Pure and Applied Chemistry, 3. 11. (2009): 228-233.

WHO. Medicinal plants. Hamdard Foundation Press, Karachi, Pakistan, (1993): 13.

Wu. Jiang ZW MantriW. BaoN. ChenXQ., S.L. and Tao ZM. “Characterizing diversity based on nutritional and bioactive compositions of yam germ plasm (Dioscorea spp.) commonly cultivated in China.” J. Food Drug Anal. 24.(2016): 367–375.doi:10.1016/j.jfda.2015.12.003.

Vimal, R.J., S.M. Charmi and B.J. Pattatiri, 2012. Pharmacognostic and scientific evaluation of the tulsi plant. Int. J. Green Her. Chem., 1(1): 75-90.

Vivek, K.G. and K.S. Surendra, 2006. Plants are natural antioxidants. Nat. Prod. Rad., 5(4): 326-334.




DOI: https://doi.org/10.21746/aps.2017.02.009



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