Phytochemical and Antioxidant activity of aqueous extract of Drypetes seperia (Putranjivaceae) fruit

Ranjani R., Madan Mohan V., Manipal K.


The study aimed to investigate phytochemical constituents and antioxidant capacity of aqueous extract of Drypetes seperia fruit pulp to testify its ethnobotanical claim. Quantification of phytochemicals, Total Phenolic Content (TPC), Total Flavonoid Content (TFC) and Antioxidant capacity determination was done by DPPH and ABTS assay. Results showed high TPC contents, TFC, and Anti-oxidant assays revealed anti-radical aptitude proving Drypetes seperia fruits as natural therapeutic agents justifying the ethnobotanical claim.


Drypetes seperia; Seshachalam; Phytochemical analysis; Antioxidant capacity


Abbas M., F. Saeed., F. M. Anjum., M. Afzaal., T. Tufail., M.S Bashir., A. Ishtiaq., S. Hussain and H. A. R Suleria. Natural polyphenols: An overview, International Journal of Food Proper-ties. 20: 8, (2017): 1689-1699.

Benzie IF, Choi SW. Antioxidants in food: content measurement, significance, action, cautions, caveats, and research needs. Advances in food and nutrition research. 71 (2014): 1-53.

Chang S K, C Alasalvar and F. Shahidi. Super-fruits: Phytochemicals, antioxidant efficacies, and health effects – A comprehensive review. Critical Reviews in Food Science and Nutrition, 59:10 (2019): 1580-1604.

Evans W.C. Trease and Evans' Pharmacognosy, 16th Edn. Saunders Ltd. (2009). 616pp. Print

Harborne J. B. Phytochemical Methods - A Guide to Modern Techniques of Plant Analysis. 2nd Edn. Springer, Dordrecht. (1998). 288 pp.

Hui Teng & Lei Chen Polyphenols and bioavailability: an update, Critical Reviews in Food Science and Nutrition, 59:13, (2019) 2040-2051

Kamiloglu S., Toydemir G., Boyacioglu D., Beekwilder J., Hall R. D., & Capanoglu E. A review on the effect of drying on antioxidant potential of fruits and vegetables. Critical Reviews in Food Science and Nutrition, 56(sup1): (2016) S129.

Kaouther Majouli1, Assia Hamdi, Malek Besbes Hlila. Phytochemical analysis and biological activities of Hertia cheirifolia L. roots extracts Asian Pacific Journal of Tropical Medicine 10(12). (2017): 1134–1139

Kurian A, Thiripuranathar G and Paranagama PA. Determination of total phenolic content and antioxidant activity of Borassus flabeliffer Linn. Fruit pulp collected from several parts of Sri Lanka. International journal of Pharmaceutical sciences and research 8(6). (2017); 2701-05.

Lutz M, Hernández J & Henríquez C. Phenolic content and antioxidant capacity in fresh and dry fruits and vegetables grown in Chile, CyTA - Journal of Food, 13:4, (2015) 541-547.

Mitta MN, Rao MS, Ramesh L and Chetty KM. Phyto-Chemical Evaluation and Anti-oxidant potentiality of Cycas beddomei Dyer Male cone aqueous Extract. International Journal of Drug Development and Research, (2014). 6 (2): 220-227.

Pereira-Netto A.B. Tropical Fruits as Natural, Exceptionally Rich, Sources of Bioactive Com-pounds, International Journal of Fruit Science, 18: 3 (2018), 231-242.

Rasouli H., Farzaei M H & Khodarahmi R. Polyphenols and their benefits: A review, International Journal of Food Properties, 20:sup2, (2017) 1700-1741.

Roghini R and Vijayalakshmi K. Phytochemical screening, quantitative analysis of flavonoids and minerals in ethanolic extract of Citrus paradisi. International Journal of Pharmaceu-tical Sciences and Research. 9(11). (2018): 4859-64.

Ruiz-Torralba A, Guerra-Hernández E J & Belen García-Villanova. Antioxidant capacity, polyphenol content and contribution to dietary intake of 52 fruits sold in Spain, CyTA - Journal of Food, 16:1, (2018) 1131-1138.

Sarmistha S, Ramtej JV. Antioxidant activity of polyphenolic extract of Terminalia chebula Retzius fruits. Journal of Taibah University for Science 10(6): 2016. 805-812

Scalbert A., Claudine Manach, Christine Morand, Christian Rémésy & Liliana Jiménez Dietary Polyphenols and the Prevention of Diseases, Critical Reviews in Food Science and Nutrition, 45:4, (2005). 287-306

Singleton VL., Orthofer R., & Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In Methods in enzymology. 299, (1999). 152-178.

Soquetta M.B., L.M Terra and C.P Bastos. Green technologies for the extraction of bioactive compounds in fruits and vegetables, CyTA - Journal of Food, 16:1. (2018) 400-412.

Soquetta M.B., Stefanello F.S., daMota Huerta K., Monteiro S. S., da Rosa C. S., & Terra N. N. Characterization of physicochemical and microbiological properties, and bioactive compounds, of flour made from the skin and bagasse of kiwi fruit (Actinidia deliciosa). Food Chemistry, 199, (2016). 471–478.

Stafussa A. P, G. M Maciel, V. Rampazzo, E. Bona, C. N Makara, B. D Junior and C.W.I Haminiuk. Bioactive compounds of 44 tradi-tional and exotic Brazilian fruit pulps: phenolic compounds and antioxidant activity. Inter-national Journal of Food Properties, 21:1, (2018): 106-118

Wang X., Li C., Liang D., Zou Y., Li P. & Ma F. Phenolic compounds and antioxidant activity in red-fleshed apples. Journal of Functional Foods, 18, (2015). 1086 – 1094

Woisky R. G. and Salatino A., “Analysis of propolis: some parameters and procedures for chemical quality control,” Journal of Apicultural Research, 37(2): (1998). 99–105.


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