Study of oncolytic enzyme production from marine Streptomyces krainskii VMB-11

Usha Y., Vishnuvardhan Z.

Abstract


L-asparaginase is an important enzyme, plays a significant role in treating ALL (Acute lymphoblastic leukemia), acute myelocytic leukemia, and other carcinoma treatments. It is well known as an anti-cancerous agent because of its stability in varied environmental conditions. Streptomyces sp. is occupied the first position for the producing L-asparaginase from marine and terrestrial sources. In the presence of L-asparaginase, tumor cells get deprived and can’t endure. It acts as a potent antitumor or anti-leukemic drug. Because of the urgent need, an attempt was made to isolate and optimize marine actinomycetes for L-asparaginase production. The S. krainskii VMB-11 was isolated and inoculated in optimized M-9 media at 40°C, 96 h improving L-asparaginase production from 8.2 IU to 13.2 IU/g dry wt. of biomass. This is often the primary report on production, purification, and characterization of L-asparaginase from marine Streptomyces isolate VMB-11 strain to the simplest of our data.

Keywords


Marine actinomycetes; Streptomyces krainskii VMB-11; L-asparaginase

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DOI: https://doi.org/10.5281/aps.2020.9.9.2

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