Analytical Quantification of mycotoxin by LC-ESI-MS/MS, and MALDI-TOF MS in phoma sorghina species of sugarcane

Abstract

Mycotoxins are secondary metabolites produced by fungi, which are harmful to humans and animals. Phomais a common fungal plant pathogen that can produce mycotoxins in sugarcane. Phoma sorgina belonging to phoma species that cause devastating fungal disease twisted leaf on sugarcane, The present  study  helps  to carry  out   research  on   potential  metabolites of  fungal  strain belongs to the phoma sorghina species such as P. sorghina (BS2-1),  P. sorghina (BS11-1),  P. sorghina  (BSQP)  isolated from sugarcane. These metabolites may influence the functioning of the fungus and are thus important indirectly for its growth and survival. Mass spectrometry (MS) is commonly used for the detection, characterization and quantitation of mycotoxins in agricultural crops. Phoma species were assessed to provide a risk assessment of their secondary metabolites in sugarcane by describing their global metabolome data obtained from the LC-MS instrument. Another study examined through using matrix-assisted laser desorption ionisation – time of flight mass spectrometry (MALDI-TOF MS) to identify phomaspecies rapidly and accurately. Different protein mass spectra obtained with MALDI-TOF MS were able to separate the phoma species reported in sugarcane. Therefore, the discovery of new conjugated mycotoxins and secondary metabolites produced phoma species was made possible by combining high mass accuracy data with analytic approaches.