Effect of Salt (NaCl) Stress on Callus Growth in Sunflower (Helianthus Annuus L.) Genotypes

Anuradha Chetukuri

Abstract


Responses of sunflower tissues to NaCl stress were studied in control and salt-stressed conditions. This study was aimed to induce callus from four sunflower (Helianthus annuus L.) genotypes, namely APSH-11, KBSH-1, PKVSH-27 and Morden and to evaluate the response of callus for salt stress. Callus was initiated from cotyledonary leaf explants of four sunflower genotypes both under salt stress and normal conditions using MS media supplemented with 1.0mg l-1 NAA in combination with 0.5 mg l-1 BAP. Callus initiation was delayed and relative callus growth decreased as the salinity level increased. Earlier callus initiation and maximum callus growth was observed in PKVSH-27 and least in Morden. This may be attributed to the genetic architecture of the genotypes. Among the salt concentration significant differences in callus growth was observed at salt (NaCl) concentration /E, C. level of 1.3 dS/m.

Keywords


Sunflower; Salt stress; Callus.

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