Identification of Bioactive Phytometabolites in Essential Oil of African Marigold (Tagetes erecta L.) Using FT-IR & DART-MS Techniques

Sandeep Kumar Rajvanshi, Deepa H. Dwivedi, Vikas Kumar

Abstract


Tagetes erecta L. belongs to family Asteraceae, most widely used as ornamental plant, has cosmetic as well as medicines values. Since oil extracted from its plant parts for secondary metabolites present in the plant such as alkaloids, steroids, tannins and phenolic compounds, flavonoids etc. Oil was extracted from flowers, as well as leaves marigold (Tagetes erecta L.) in fresh form and after processing by solvent extraction method using n-hexane as solvent. Samples were subjected to Fourier transform infrared spectroscopy (FT-IR) where spectra of samples have shown the presence of major functional groups viz., alkanes, aromatic, phenols, aliphatic, nitro compounds and amines, etc at wavelength of 3400 to 3480 cm-1 (alcohol & phenols), 2850 to 2990 cm-1 (alkanes), 1050 to 1200 cm-1 (aliphatic amines) and 720 to 890 cm-1 (aromatics). Presence of more number of peaks shows the presence of more number of bioactive compounds. These samples were further validated by DART Mass Spectrometry where peak at m/z 136 correspond to terpenoids, 151 (ketone), 169 (phenols), 183 (alcohol) etc. Presence of phenolic compound galic acid in the samples proves the antioxidant activity of the samples

Keywords


Bioactive compounds, Solvent extraction, African marigold, Tagetes oil, DART-MS and FT-IR

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