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Standardization and re-introduction of Critically Endangered Ceropegia mahabalei Hemadri and Ansari by in vitro propagation

Anuradha S. Upadhye*, Priyanka B. Waghamode, Pallavi M. Dhavare, Namrata S Gaikwad

Abstract


A protocol is described for micropropagation of the tuberous, herbaceous, medicinally important and Critically Endangered Ceropegia mahabalei Hemadri and Ansari by in vitro culture of nodal segments. The effect of cytokinins (BAP, Kin and TDZ) and auxins (IBA, IAA and NAA) at various concentrations on in vitro regeneration were investigated. The highest number of nodes per explant (7.5±0.14) and maximum shoot length (8.02±0.12 cm) were found on full strength MS medium supplemented with BAP (8.88 µM). Whereas, IBA (9.84 µM) showed maximum root length (1.54±0.09 cm) and number of roots per shoot (5.8±0.37) with maximum shoot length (7.11±0.50cm). In vitro grown plantlets were acclimatized (100%) in potting material containing mixture of garden soil, coco peat and river sand (1:1:1/2) and showed normal development with flowering. The developed method can be employed for productive conservation of C. mahabalei through large scale in vitro propagation. These saplings were successfully reintroduced in their natural habitat with a survival rate of 42.67 %.

Keywords


Ceropegia mahabalei; Critically Endangered; Micropropagation; Reintroduction

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