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Meristem culture for rapid regeneration in Black pepper (Piper nigrum Linn.)

Umadevi P.*, K. V. Saji, E. J. Suraby

Abstract


The shoot apical meristem for production of plantlets from black pepper variety Sreekara was assessed. Meristem extension was achieved in liquid medium containing Murashige and Skoog with 0.1 mgl-1 kinetin and 0.5 mgl-1 GA3, subsequent direct shoot induction in ½ MS with 3 mgl-1 BA and 1 mgl-1 IAA followed by shoot growth and development in ½ MS +0.5 mgl-1Indole butyric acid. The meristems of 2mm resulted in shoot induction and proliferation via direct organogenesis. Successful rooting of meristem derived shoots was achieved in half strength WPM with 3 mgl-1 BA and 1 mgl-1 Kinetin. In vitro multiplication from the meristem derived plants shows the possibility for mass multiplication. The developed protocol was effective with 40% regeneration frequency of meristems, simple and rapid protocol for regeneration with 66-70days and subsequent micro propagation for the production of plant stock. This is the very first report on meristem culture in blackpepper. The protocol will be of immense importance in rapid mass multiplication of elite germplasm, as well as for conservation of this important species.

Keywords


Blackpepper; Meristem culture; Direct organogenesis.

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References


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